Table of Contents
ISRN Genetics
Volume 2013, Article ID 471632, 14 pages
Research Article

Construction of Genetic Linkage Map and QTL Analysis of Sink-Size Traits in Pearl Millet (Pennisetum glaucum)

1Pandit Jawaharlal Nehru College of Agriculture and Research Institute, Karaikal, Pondicherry 609 603, India
2International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh 502 324, India
3Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003, India
4ICRISAT Sahelian Center, BP 12404, Niamey, Niger
5International Institute of Tropical Agriculture, PMB 5320, Ibadan, Oyo State, Nigeria
6Directorate of Oilseeds Research, Indian Council of Agricultural Research, Hyderabad, Andhra Pradesh 500 030, India
7International Maize and Wheat Improvement Center, ICRISAT, Patancheru, Andhra Pradesh 502 324, India
8Indian Agriculture Research Institute, New Delhi 10 012, India

Received 27 August 2013; Accepted 4 November 2013

Academic Editors: M. Falk, J. Leips, and D. G. Peterson

Copyright © 2013 V. Vengadessan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A linkage map, primarily based on SSCP-SNP markers, was constructed using 188 F2:3 mapping population progenies produced from a cross between two pearl millet inbred lines having diverse parentage. The skeleton linkage map covered 1019 cM and it comprised of 44 markers distributed across the seven linkage groups. Average adjacent-marker intervals ranged from 14 cM on LG1 to 38 cM on LG6, with an overall mean of 23 cM. Using the F2 linkage map and phenotypic data from the F2 and F2:3 generations of the mapping population, a total of 18 putative QTLs were detected for the three sink-size components. Eight QTLs explained 42.7% of observed phenotypic variation for panicle length using the F2:3 data set. For panicle diameter, 5 QTLs explained 45.8% of observed phenotypic variation. Similarly for grain size, 5 QTLs explained 29.6% of phenotypic variation. Genomic regions associated with panicle length, panicle diameter, and grain size were comapped on LG6 between Xpsms88 and Xpsms2270, indicating the existence of a gene or gene cluster. The QTLs for panicle length on LG2 and LG6 ( in both F2 and F2:3 data sets), for panicle diameter on LG2 and LG3 ( in the F2:3 data set), and for grain size on LG3 and LG6 ( in both F2 and F2:3 data sets) were identified as promising candidates for validation prior to possible application in marker-assisted breeding.