Research Article

Microsatellite Instability in Head and Neck Squamous Cell Carcinoma: A Study of a Brazilian Population

Table 2

Genomic characteristics and reaction conditions for MSI markers.

CharacteristicsMSI marker
D3S1611D5S346D10S197D11S922D11S988D17S250

Genomic
 Chromosomal location3p21.35q2110p1211p15.511p15.517q11.2
 STR sequence
 Fragment size140 pb115 pb141 pb175 pb120 pb151 pb
Reaction conditions
 Platinum taq DNA polymerase0.2 u0.2 u0.2 u0.2 u0.2 u0.2 u
 25 µM primers F/R0.3 µL0.6 µL0.6 µL0.6 µL0.6 µL0.6 µL
 10 mM dNTP0.2 mM0.2 mM0.2 mM0.2 mM0.2 mM0.2 mM
 50 mM MgCl21.5 mM1.5 mM1.5 mM1.5 mM1.5 mM1.5 mM
 10X PCR buffer1X1X1X1X1X1X
 DMSO 100%0.75 µL
 DNA1.2 µL1.2 µL0.6 µL1.8 µL1 µL0.6 µL
 Final volume15 µL15 µL15 µL15 µL15 µL15 µL
Cycling conditions
 Number of cycles303028303230
 Annealing70°C for 15seg55°C; 30seg70°C; 30seg68°C; 30seg69°C; 30seg50°C; 30seg