Research Article
Microsatellite Instability in Head and Neck Squamous Cell Carcinoma: A Study of a Brazilian Population
Table 2
Genomic characteristics and reaction conditions for MSI markers.
| Characteristics | MSI marker | D3S1611 | D5S346 | D10S197 | D11S922 | D11S988 | D17S250 |
| Genomic | | | | | | | Chromosomal location | 3p21.3 | 5q21 | 10p12 | 11p15.5 | 11p15.5 | 17q11.2 | STR sequence | | | | | | | Fragment size | 140 pb | 115 pb | 141 pb | 175 pb | 120 pb | 151 pb | Reaction conditions | | | | | | | Platinum taq DNA polymerase | 0.2 u | 0.2 u | 0.2 u | 0.2 u | 0.2 u | 0.2 u | 25 µM primers F/R | 0.3 µL | 0.6 µL | 0.6 µL | 0.6 µL | 0.6 µL | 0.6 µL | 10 mM dNTP | 0.2 mM | 0.2 mM | 0.2 mM | 0.2 mM | 0.2 mM | 0.2 mM | 50 mM MgCl2 | 1.5 mM | 1.5 mM | 1.5 mM | 1.5 mM | 1.5 mM | 1.5 mM | 10X PCR buffer | 1X | 1X | 1X | 1X | 1X | 1X | DMSO 100% | | 0.75 µL | | | | | DNA | 1.2 µL | 1.2 µL | 0.6 µL | 1.8 µL | 1 µL | 0.6 µL | Final volume | 15 µL | 15 µL | 15 µL | 15 µL | 15 µL | 15 µL | Cycling conditions | | | | | | | Number of cycles | 30 | 30 | 28 | 30 | 32 | 30 | Annealing | 70°C for 15seg | 55°C; 30seg | 70°C; 30seg | 68°C; 30seg | 69°C; 30seg | 50°C; 30seg |
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