Delayed Asthmatic Response to Allergen Challenge and Cytokines Released by Nonspecifically Stimulated Blood Cells
Table 5
Mean values of cytokine concentrations in the supernatants (pg/mL) released from peripheral blood cells stimulated “in vitro” with various nonspecific stimulating agents.
Control subjects
DYAR,
Asthmatics
Healthy subjects
PHA/PMA/fMLP
IAR,
LAR,
DLAR,
PHA/PMA/fMLP
PHA/PMA/fMLP
PHA/PMA/fMLP
PHA/PMA/fMLP
IL-1
7.1/7.4/6.8
7.5/7.3/7.0
7.4/7.7/7.5
8.2/8.1/8.4
8.5/8.3/8.0
IL-2
4.2/3.9/4.0
*
<3.0
<3.0/3.2/<3.0
<3.0
<3.0
IL-3
<31.2
<31.2
33.2/33.5/<31.2+
<31.2
<31.2
IL-4
19.8/21.2/20.0
33.8/35.9/34.7
*
24.0/23.5/23.9
31.0/32.8/33.0
*
21.6/22.9/21.8
IL-5
5.1/6.0/5.7
8.7/9.5/9.3
*
9.4/10.6/9.8
*
9.5/11.0/9.7
*
4.9/5.0/4.3
IL-6
4.6/5.0/5.2
6.4/7.1/7.2
*
4.7/5.2/5.0
7.3/7.2/8.0
*
4.0/4.8/3.7
IL-7
3.3/4.2/4.1
8.9/10.0/9.4
*
3.5/3.5/3.8
4.1/4.4/3.4
3.9/3.5/3.6
IL-8
9.6/10.3/9.0+
7.4/7.1/7.9
10.5/11.0/10.6
*
9.5/12.1/11.3*
8.2/7.0/6.5
IL-10
8.1/9.0/8.5
12.6/12.2/12.7
*
8.3/7.6/7.9
8.1/8.0/7.9
7.7/8.4/8.2
IL-12p40
9.8/10.5/10.5
13.9/14.4/14.2+
15.6/15.0/15.3
*
14.2/16.7/16.5
*
10.1/9.6/9.5
IL-12p70
13.0/14.9/13.2+
14.0/14.8/14.6+
10.3/9.9/10.1
12.0/12.6/11.7
11.5/11.9/11.8
IL-13
3.8/<3.0/<3.0
10.1/10.7/9.8
*
9.9/10.3/10.0
*
11.0/11.5/10.8
*
3.4/4.0/<3.0
IL-18
19.6/20.0/20.0
*
14.1/13.9/13.7
15.2/15.5/15.0
16.1/15.7/15.5
15.9/16.5/14.2
IFN-
373/395/350
*
199/207/183
191/212/205
213/218/211
224/237/255
GM-CSF
10.4/10.5/9.6
12.7/14.0/13.9
*
13.5/14.3/13.8*
15.4/15.0/14.6
*
10.0/9.4/7.0
G-CSF
7.0/7.4/6.8
*
3.9/4.5/4.5
3.7/3.5/3.1
4.0/4.4/3.6
4.1/3.5/3.2
TNF-
11.7/12.9/11.3+
8.5/8.9/9.1
10.4/11.7/11.6+
7.8/7.5/7.6
9.0/8.8/7.9
TGF-
16.8/17.5/17.4
17.5/17.1/18.0
20.4/22.6/21.9+
23.0/23.7/22.8
*
18.0/18.2/16.7
DYAR: delayed asthmatic response; IAR: immediate asthmatic response; LAR: late asthmatic response; DLAR: dual late asthmatic response. Values = means ± SEM. Statistical significance as compared with healthy subject values: *; + (borderline). PHA: Phytohemagglutin in (10 g/mL); PMA: Phorbol 12-myristate 13-acetate (50 ng/mL); fMLP: N-formylmethionyl-leucyl-phenylalanine (1 mol/L).
