Table of Contents
ISRN Allergy
Volume 2013 (2013), Article ID 509427, 7 pages
http://dx.doi.org/10.1155/2013/509427
Research Article

Identification of T- and B-Cell Subsets That Expand in the Central and Peripheral Lymphoid Organs during the Establishment of Nut Allergy in an Adjuvant-Free Mouse Model

1Food Allergy and Immunology Laboratory, Michigan State University, East Lansing, MI 48823, USA
2Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48823, USA
3Division of Applied Life Science (BK 21 Program), Gyeongsang National University, Jinju, Republic of Korea

Received 25 February 2013; Accepted 15 March 2013

Academic Editors: S. Burastero, B. F. Gibbs, and R. Paganelli

Copyright © 2013 Babu Gonipeta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nut allergies are potentially fatal and rarely outgrown for reasons that are not well understood. Phenotype of T- and B-cell subsets that expand during the early stages of nut allergy is largely unknown. Here we studied this problem using a novel mouse model of nut allergy. Mice were rendered hazelnut allergic by a transdermal sensitization/oral elicitation protocol. Using flow cytometry, the T- and B-cell phenotype in the bone marrow (BM), spleen, and the mesenteric lymph node (MLN) of allergic and control mice was analyzed. Nut allergic mice exhibited an expansion of CD4+ CD62L− T cells in BM and spleen; a similar trend was noted in the MLN. There was expansion of CD80+ B cells in BM and spleen and MLN and CD62L− cells in BM and spleen. Interestingly, among CD80+ B cells, significant proportion was CD73− particularly in the MLN. These data demonstrate that during the early establishment of hazelnut allergy there is (i) expansion of CD4+CD62L− T-cell subsets in both the BM and the periphery, (ii) expansion of CD80+ and CD62L− B-cell subsets in BM and the periphery, and (iii) a significant downregulation of CD73 on a subset of B cells in MLN.