Table of Contents
ISRN Biotechnology
Volume 2013, Article ID 590587, 7 pages
http://dx.doi.org/10.5402/2013/590587
Review Article

Critical Factors Affecting the Success of Cloning, Expression, and Mass Production of Enzymes by Recombinant E. coli

1Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh
2BCSIR Laboratories, Chittagong, Chittagong 4220, Bangladesh
3Center for Food and Waterborne Diseases (CFWD), ICDDRB, Dhaka, Bangladesh

Received 1 July 2012; Accepted 7 August 2012

Academic Editors: G. Ladics and J. J. Valdes

Copyright © 2013 Md. Fakruddin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

E. coli is the most frequently used host for production of enzymes and other proteins by recombinant DNA technology. E. coli is preferable for its relative simplicity, inexpensive and fast high-density cultivation, well-known genetics, and large number of compatible molecular tools available. Despite all these advantages, expression and production of recombinant enzymes are not always successful and often result in insoluble and nonfunctional proteins. There are many factors that affect the success of cloning, expression, and mass production of enzymes by recombinant E. coli. In this paper, these critical factors and approaches to overcome these obstacles are summarized focusing controlled expression of target protein/enzyme in an unmodified form at industrial level.