Figure 1: hSpry1 colocalizes with uPAR in cells with stimulated uPA and uPA-ATF. (a) HEK293/uPAR and (b) MDA-MB-231 cells overexpressing hSpry1 were kept as negative control (A) or serum-starved overnight (B) and then treated with 100 ng/mL of either EGF (C), uPA (D), or uPA-ATF (E) for 20 min. Cells were fixed and then immunostained with antibodies against uPAR (red) and hSpry1 (green), and colocalization appeared as a yellow colour. These cells were analysed using confocal laser scanning microscope and 60x oil immersion lens (final magnification 600x). Data are representative of 3 independent experiments.