Protective Effects of Guava Pulp on Cholestatic Liver Injury
Effect of guava pulp on gene expression. (a) The phosphor-receptor tyrosine kinase (Phosphor-RTK) array. Cytoplasmic proteins from the ligated lobes in LMBDL and LMBDL + guava groups. Arrow no. 1, no. 2, no. 3, no. 4, no. 5, and no. 6 represented phosphor-p38α, phosphor-ERK1/2, phosphor-JNK, phosphor-β-catenin and phosphor-Src, respectively. (b) Densitometric analysis of p-p38α, pERK, pMEK, pJNK, p-β-catenin, and pSrc in the ligated lobes of LMBDL and LMBDL + guava. (c) Representative Western blots for the expression level of pERK, total ERK, pMEK, total MEK, pSrc, and total Src in the ligated lobes in LMBDL and LMBDL+GP groups. (d) Quantitative assays of densitometric changes expressed as percentage of phosphorylation over total protein. A total of 24 animals (6 per group) were studied at day 28. , percentage of pERK/total ERK in LMBDL+GP group versus percentage of pERK/total ERK in LMBDL group, , percentage of pSrc/total Src in LMBDL+GP group versus percentage of pSrc/total Src in LMBDL group, , percentage of pMEK/total MEK in LMBDL+GP group versus percentage of pMEK/total MEK in LMBDL group. (e) Determination and quantification of c-Myc positive biliary epithelial cells. the ligated lobes of LMBDL+GP groups at days 1, 7, and 28 versus the ligated lobes of LMBDL groups at 1, 7, and 28, respectively.