Research Article

Protective Effects of Guava Pulp on Cholestatic Liver Injury

Figure 5

Effect of guava pulp on QBC939 cell growth, p-ERK, and c-Myc gene expression. (a) BrdU assay of QBC939 cells after treatment with P. guajava L. pulp extract for 24 h. , the groups at concentration of 0.4 mg/mL, 0.8 mg/mL and 1.6 mg/mL versus control. Con = control (0 mg/mL). (b) QBC939 cells treated with IL-6 and GP. QBC939 cells were treated with IL-6 at concentration of 0.5, 5, 10, 20 and 40 (ng/mL) for 24 h. , control (Con) versus groups of 2.5, 5, 10, and 20 IL-6 (ng/mL) treatment, respectively. GP treatment significantly reduced IL-6-induced QBC939 cell proliferation. ,  IL-6 treatments versus IL-6+GP treatment. Proliferation was assessed using BrdU cell proliferation assay. (c) Representative Western blots for the expression level of pERK and c-Myc at 24 h with or without treatment with GP extract and IL-6. Quantitative assays of densitometric changes expressed as percentage of phosphorylation over total protein QBC939 cells at 24 h after treatment with GP extract (0.8 mg/mL). , control versus IL-6 or IL-6+GP treatment, ,  IL-6 treatment versus IL-6+GP treatment.