Table of Contents
ISRN Hepatology
Volume 2013, Article ID 601071, 11 pages
Research Article

Protective Effects of Guava Pulp on Cholestatic Liver Injury

1National Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital of Central South University, 87 Xiangya Road, Changsha, Hunan 410008, China
2Surgery Department, School of Medicine, Complutense University, Madrid, Spain
3Department of Nutritional Science and Food Management, The College of Health Science, Ewha Womans University, Seoul, Republic of Korea
4Division of Gastroenterology and Liver Diseases, USC Research Center for Liver Diseases, Department of Medicine, Keck School of Medicine, USC, HMR Building, 415, 2011 Zonal Avenue, Los Angeles, CA 90033, USA

Received 5 August 2013; Accepted 5 September 2013

Academic Editors: S. DeMorrow, H. Denk, M. G. Mancino, and S. Pinlaor

Copyright © 2013 Jian Peng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure: Procedure of LMBDL surgery. A) Ketamine (100 mg/kg/BW) with Xylazine (5 mg/kg/BW) was intraperitoneally injected into the lower right quadrant of the mouse. B) Hair was removed from the operative site. C) The mouth of the mouse was gently prying opened with forceps, and then the tongue was pulled out and held to the right side. D) The abdomen skin was cleaned by swabbing with 70% ethanol solution followed by povidone iodine solution to prevent bacterial infection during the LMBDL surgical procedure. Then, laparotomy was performed to expose the abdominal contents with a small pair of scissors. The incision about 3 cm long started at the midabdomen and ended at the xiphoid process. E) Two pieces of gauze were moistened (sterile 0.9% saline) and placed on the right side of the incision (left side of mouse). Three pieces of moistened cotton gauzes were used to lift the left lobe onto the skin of the left abdomen. F) The median lobe onto the skin of the right abdomen. G) The bile duct and associated structures were exposed. Upper margin of the right lobe was determined and the portal triad (bile duct, portal vein and hepatic artery) was revealed carefully. The key step was isolation of the hepatic bile duct between the mergence of the left and median lobe and the mergence of the right and caudate lobe. H,I) The isolated hepatic bile duct was ligated with a 6-0 silk suture. Since the fluxed passages of bile in median and left lobes (approximately 70% of the liver) were blocked, the ligated bile duct would soon be full of bile. After the isolated bile duct to the gall bladder was ligated, the gallbladder was removed to avoid cholecystitis. The peritoneum was closed with a 6-0 silk suture.

  1. Supplementary Figure