Determination of Helicobacter pylori Virulence Genes in Gastric Biopsies by PCR

Table 1

Primers used for the amplification of H. pylori genes.

Primer	5′-3′ sequence	Product size (bp)	Annealing (°C)
CagA-F	AATACACCAACGCCTCCAAG	400	55
CagA-R	TTGTTGGCGCTTGCTCTC
VacA-3.AS	GCCGATATGCAAATGAGCCGC	678	66
VacA-1.SE	CAATCGTGTGGGTTCTGGAGC
IceA1-F	CGTTGGGTAAGCGTTACAGAATTT	558	56
IceA1-R	TCATTGTATATCCTATCATTACAAG
IceA2-F	GTTGTCGTTGTTTTAATGAA	120	50
IceA2-R	GTCTTAAACCCCACGATTAAA
HPU1	GCCAATGGTAAATTAGTT	411	45
HPU2	CTCCTTAATTGTTTTTAC
GlmM-F	AAGCTTTTAGGGGTGTTAGGGGTTT	294	55
GlmM-R	AAGCTTACTTTCTAACACTAACGC