Stability Indicating HPTLC Method for Analysis of Rifaximin in Pharmaceutical Formulations and an Application to Acidic Degradation Kinetic Study

Table 5

Robustness study of developed HPTLC method.

Parameters	SD^a	(%) RSD
Mobile phase composition (4.9 : 3.9 : 1 : 1, 5.1 : 4.1 : 0.9 : 0.9)	1.31	1.36
Saturation time (20±5 min)	0.50	0.50
Development distance (8±1 cm)	1.18	1.16
Scanning time interval (1 h, 2 h, 3 h, 4 h, 5 h, and 6 h)	0.15	0.15
Wavelength change (442±1 nm)	0.72	0.73
Time from spotting to chromatography (0, 20, 40, and 60 min)	0.52	0.52
Mobile phase volume (11±2 mL)	1.58	1.58

^a𝑛=3 replicates for concentration of 800 ng/band.