Table of Contents
ISRN Endocrinology
Volume 2013, Article ID 638028, 13 pages
http://dx.doi.org/10.1155/2013/638028
Research Article

n5-STZ Diabetic Model Develops Alterations in Sciatic Nerve and Dorsal Root Ganglia Neurons of Wistar Rats

1Laboratory of Electrophysiology, Superior Institute of Biomedical Sciences, State University of Ceará, Avenue Paranjana, 1700 Campus of Itaperi, 60740-903 Fortaleza, CE, Brazil
2Department of Clinical Medicine, Federal University of Ceará, Street Prof. Costa Mendes, 608 Campus Porangabuçu, 60.430-140 Fortaleza, CE, Brazil

Received 29 November 2012; Accepted 18 December 2012

Academic Editors: S. Bertera and S. Mishra

Copyright © 2013 Francisco Walber Ferreira-da-Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

One experimental model of diabetes mellitus (DM) similar to type II DM, called n5-STZ, is obtained by a single injection (via i.p.) of streptozotocin (STZ) in the 5th day of life of newborn rats. The present investigation aimed to characterize alterations in excitability of rat peripheral neurons in n5-STZ model. n5-STZ DM was induced, and electrophysiological evaluation was done at 12th week of rat life. Rats developed glucose intolerance, sensory alteration, and hyperglycemia or near-normoglycemia ( and  mmol/L). In near-normoglycemia group the significant electrophysiological alteration observed was decreased in amplitude of 2nd wave (2nd component, conduction velocity: 48.8 m/s) of compound action potential (CAP) of sciatic nerve. For hyperglycemic rats, decreased excitability, amplitude, and conduction velocity of 2nd CAP component of sciatic nerve were found; a depolarization of resting potential (4-5 mV) and reduction in maximum ascendant and descendant inclinations of action potential were found in DRG neurons but no alteration on Na+ current ( ). Thus, n5-STZ rats develop alterations in excitability which were related to glycemic levels but were not likely attributable to changes on . Our data confirm that n5-STZ model is a useful model to study type II DM.