Table of Contents
ISRN Stem Cells
Volume 2013 (2013), Article ID 674671, 9 pages
http://dx.doi.org/10.1155/2013/674671
Research Article

Comparative Analysis of Mesenchymal Stromal Cells Biological Properties

1Fibroblast Reprogramming Unit, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy
2Stem Cell Lab-SIMT, A.O. Spedali Civili, Piazzale Spedali Civili 1, 25123 Brescia, Italy
3Biostatistics Unit, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy

Received 25 January 2013; Accepted 28 February 2013

Academic Editors: A. Chapel, I. E. Hoefer, S. M. Hwang, and B. Rogister

Copyright © 2013 Angela De Luca et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The stromal progenitors of mesodermal cells, mesenchymal stromal cells (MSCs), are a heterogeneous population of plastic adherent fibroblast-like cells with extensive proliferative capacity and differentiation potential. Human MSCs have now been isolated from various tissues including bone marrow, muscle, skin, and adipose tissue, the latter being one of the most suitable cell sources for cell therapy, because of its easy accessibility, minimal morbidity, and abundance of cells. Bone marrow and subcutaneous or visceral adipose tissue samples were collected, digested with collagenase if needed, and seeded in Iscove's medium containing 5% human platelet lysate. Nonadherent cells were removed after 2-3 days and the medium was replaced twice a week. Confluent adherent cells were detached, expanded, and analyzed for several biological properties such as morphology, immunophenotype, growth rate, senescence, clonogenicity, differentiation capacity, immunosuppression, and secretion of angiogenic factors. The results show significant differences between lines derived from subcutaneous fat compared to those derived from visceral fat, such as the higher proliferation rate of the first and the strong induction of angiogenesis of the latter. We are convinced that the identification of the peculiarities of MSCs isolated from different tissues will lead to their more accurate use in cell therapy.