Comparative Analysis of Mesenchymal Stromal Cells Biological Properties
Figure 2
Differentiation capacity of adipose-derived MSC. (a) Cells were detached with trypsin and SSEA4 membrane protein was evaluated by flow cytometry using a PE-conjugated antibody. (b) qPCR analysis of the expression of pluripotency markers in MSC. Total RNA was extracted from cell lines, equivalent amounts of cDNA were amplified by qPCR and gene expression levels were quantified using REST [10]. BM-MSC was used as a reference cell line. Data are representative of at least two independent experiments. (c) Oil Red O staining of MSC after adipogenic induction, Alcian Blue staining after chondrogenic induction, and Alizarin Red staining after osteogenic induction.
