Clinical Study
Cell Proliferation (KI-67) Expression Is Associated with Poorer Prognosis in Nigerian Compared to British Breast Cancer Women
Table 1
Sources, dilution, distribution, cut-offs point, and pretreatment used for revalidation.
| Antibody | Clone | Source | Dilution | Distribution | Scoring System | Cut-offs | Pretreatment | Positive control | Negative control |
| Bcl-2 | 124 | Dako-Cytomation | 1 : 100 | Cytoplasm | % of positive cells | >10% (positive) | Antigen retrieval microwave | Normal breast acini | Omitting the antibody | BRCA1 | Ab-1 (MS110) | Calbiochem | 1 : 150 | Nuclear | % of positive cells | <25% (negative)
| Antigen retrieval microwave | MCF 7 cells | Omitting the antibody | Ck5/6 | M7237 | Dako-Cytomation | 1 : 60 | Cytoplasm | % of positive cells | ≥10% (positive) | Antigen retrieval microwave | Known case of CK56 BC | Omitting the antibody | E-cadherin | NCH-38 | Dako-Cytomation | 1 : 100 | Cytoplasm and membrane | % of positive cells | ≥100 H score (positive) | Antigen retrieval microwave | Normal gastric mucosa | Omitting the antibody | EGFR | 31G7 | Novocastra | 1 : 30 | Membrane | % of positive cells | ≥10% (positive) | Not required | Myoepithelial cells of normal duct in normal mammary gland | Omitting the antibody | erbB2 | Polyclonal | Dako-Cytomation | 1 : 100 | Membrane | Table 2.1 | Table 2.1 | Not required | Known case of erbB2 strong BC expression | Omitting the antibody | ER | 1D5 | Dako-Cytomation | 1 : 200 | Nuclear | % of positive cells | ≥0 (positive) | Antigen retrieval microwave | Normal breast acini | Omitting the antibody | Ki-67 | MIB1 | Dako-Cytomation | 1 : 25 | Nuclear | % of positive cells | <10% (low)
| Antigen retrieval microwave | Human tonsil tissue | Omitting the antibody | P-cadherin | NCL-P-cad
| Novocastra
| 1 : 200 | Cytoplasm | % of positive cells | ≥5% (positive) | Antigen retrieval microwave | Known case of P-cadherin strong BC expression | Omitting the antibody | PgR | PgR | Dako-Cytomation | 1 : 150 | Nuclear | % of positive cells | ≥0 (positive) | Antigen retrieval microwave | Normal breast acini | Omitting the antibody | p21 | EA10 | Abcam | 1 : 25 | Nuclear | % of positive cells | ≥10% (positive) | Antigen retrieval microwave | Normal breast acini | Omitting the antibody | p53 | DO7 | Novocastra | 1 : 50 | Nuclear | % of positive cells | >10% (negative)
| Antigen retrieval microwave | Normal breast acini | Omitting the antibody |
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