Subcutaneous Administration of D-Luciferin is an Effective Alternative to Intraperitoneal Injection in Bioluminescence Imaging of Xenograft Tumors in Nude Mice
Figure 1
Potential for missed injection when D-luciferin is administered IP. (a) OSC19-Luc tongue xenografts were established in 4 nude mice, and BLI was performed after IP injection of D-luciferin. BLS intensity was evaluated for 1 min at 7 min after injection. Mouse no. 3 (*) exhibits no BLS due to inadvertent intraintestinal injection. (b) Mouse no. 3 (*) underwent a second IP administration of D-luciferin and repeat BLI was performed. Mouse no. 3 (*) exhibited the expected BLS after successful reinjection. (c) A single nude mouse bearing a subcutaneous U87-Luc xenograft underwent BLI after deliberate intraintestinal injection of D-luciferin. BLS was collected continuously at 1 min intervals as described in Methods. After 3 h, correct IP injection D-luciferin was performed, and BLS was collected as before. The graph shows BLS intensity over time for the intestinal (INT) and IP injections.
