Antileukemic Efficacy of Monomeric Manganese-Based Metal Complex on KG-1A and K562 Cell Lines
(a) Intracellular reduced glutathione (GSH) levels of MMTC treated KG-1A and K562 cell lines. The levels of GSH were expressed as μg of GSH mg−1 protein. (b) Intracellular oxidized glutathione (GSSG) levels of MMTC treated KG-1A and K562 cell lines. The levels of GSSG were expressed in terms of μg of GSSG mg−1 protein. Nitric oxide (NO) release levels of MMTC treated KG-1A and K562 cell lines. (c) The levels of NO were expressed as μmol mg−1 protein. (d) Effects of MMTC on ROS induction in KG-1A and K562 cell lines. Data is represented as the percentage of the ROS level in the control group. Values are expressed as mean ± SEM of three experiments; * and # indicate significant difference () compared to the control group. (D (i), D (ii), D (iii), and D (iv)) Qualitative characterization of reactive oxygen species formation by DCFH2-DA staining using fluorescence microscopy. Here, D (i): Control KG-1A cells; D (ii): MMTC treated KG-1A cells; D (iii): Control K562 cells; D (iv): MMTC treated K562 cells.
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