Examination of the Anti-Inflammatory, Antioxidant, and Xenobiotic-Inducing Potential of Broccoli Extract and Various Essential Oils during a Mild DSS-Induced Colitis in Rats

Table 3

Gene bank accession numbers and primer sequences of the genes investigated by real-time RT-PCR.

Gene name (abbreviation used)	Gene bank accession number	Primer sequences (5^′→3^′); for = forward; rev = reverse;
Chemokine (C-C motif) ligand 2 (Ccl2) (MCP1)	NM_031530	for: GTGCGACCCCAATAAGGAA
		rev: TGAGGTGGTTGTGGAAAAGA
Claudin 3 (Cldn3)	NM_031700	for: TATCCTACTGGCAGCCTTCG
		rev: GTTCCCATCTCTCGCTTCTG
Copper/zinc superoxide dismutase (SOD1)	NM_017050	for: CCACTGCAGGACCTCATTTT
		rev: CACCTTTGCCCAAGTCATCT
C-reactive protein (CRP)	NM_017096	for: GTCTCTATGCCCACGCTGAT
		rev: CCGTCAAGCCAAAGCTCTAC
Glutathione S-transferase K1 (GSTK1)	NM_181371	for: GAGCATGGAGCAACCAGAGAT
		rev: AGCTTGCTCTTCACCAGTTCG
Glutathione S-transferase P1 (GSTP1)	NM_012577	for: GAGGCAAAGCTTTCATTGTGG
		rev: GTTGATGGGACGGTTCAAATG
Glutathione S-transferase T2 (GSTT2)	NM_012796	for: GAGGAAAAGGTGGAACGGAAC
		rev: CGCCCCTCAAACAGATTACAG
Glutathione peroxidase 2 (GPx2)	NM_183402	for: GTGTGATGTCAATGGGCAGAA
		rev: ACGTTTGATGTCAGGCTCGAT
Heme oxygenase 1 (HO1)	NM_012580	for: AGGCACTGCTGACAGAGGAAC
		rev: AGCGGTGTCTGGGATGAACTA
Hypoxanthine phosphoribosyltransferase 1 (Hprt1)	NM_012583	for: GCAGACTTTGCTTTCCTTGG
		rev: TCCACTTTCGCTGATGACAC
Interleukin 1 beta (IL-1β)	NM_031512	for: CTGTGACTCGTGGGATGATG
		rev: GGGATTTTGTCGTTGCTTGT
Interleukin 10 (IL-10)	NM_012854	for: CTGGAGTGAAGACCAGCAAAGG
		rev: GGAGAAATCGATGACAGCGTCG
Kelch-like ECH-associated protein1 (Keap1)	NM_057152	for: GTGGCGGATGATTACACCAAT
		rev: GAAAAGTGTGGCCATCGTAGC
NAD(P)H dehydrogenase [quinone] 1 (NQO1)	NM_017000	for: CGCAGAGAGGACATCATTCA
		rev: CGCCAGAGATGACTCAACAG
Nuclear factor (erythroid-derived 2)-like 2 (Nrf2)	NM_031789	for: CCAAGGAGCAATTCAACGAAG
		rev: CTCTTGGGAACAAGGAACACG
Nuclear factor kappa B (NFκB)	L26267	for: CTTCTCGGAGTCCCTCACTG
		rev: CCAATAGCAGCTGGAAAAGC
Prostaglandin-endoperoxide synthase 2 (Ptgs2) (COX2)	NM_017232	for: GCTGTACAAGCAGTGGCAAA
		rev: CCCCAAAGACAGCATCTGGA
Ribosomal protein L13A (Rpl13a)	NM_173340	for: CCCTCCACCCTATGACAAGA
		rev: CCTTTTCCTTCCGTTTCTCC
Tumor necrosis factor alpha (TNFα)	NM_012675	for: GCCAATGGCATGGATCTCAAAG
		rev: AAATCGGCTGACGGTGTGGG
Vascular cell adhesion molecule 1 (VCAM 1)	NM_012889	for: TGACATCTCCCCTGGATCTC
		rev: CTCCAGTTTCCTTCGCTGAC
β-actin	NM_031144	for: ATCGTGCGTGACATTAAAGAGAAG
		rev: GGACAGTGAGGCCAGGATAGAG