Adipose-Derived Stem Cells in Tissue Regeneration: A Review

Figure 1

ASC isolation and utilization. Schematic outline of the isolation of ASCs from lipoaspirates showing the enzymatic digestion of adipose tissue with collagenase type IA and the isolation of the SVF via centrifugation. Plating of the SVF results in eventual selection and expansion of the ASC population. Induction of ASCs under defined conditions can result in their differentiation to multiple cell types of the mesodermal (bone, fat, cartilage, muscle types), ectodermal (endothelium, neurons, epidermis/skin), and endodermal (liver) lineages.