Table of Contents
ISRN Biotechnology
Volume 2013 (2013), Article ID 718352, 5 pages
http://dx.doi.org/10.5402/2013/718352
Research Article

Application of Plackett-Burman Experimental Design for Lipase Production by Aspergillus niger Using Shea Butter Cake

1Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
2Department of Biochemistry, Bayero University, Kano, Nigeria

Received 6 December 2012; Accepted 10 January 2013

Academic Editors: L. Betancor and J. J. Valdes

Copyright © 2013 Aliyu Salihu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Plackett-Burman design was used to efficiently select important medium components affecting the lipase production by Aspergillus niger using shea butter cake as the main substrate. Out of the eleven medium components screened, six comprising of sucrose, (NH4)2SO4, Na2HPO4, MgSO4, Tween-80, and olive oil were found to contribute positively to the overall lipase production with a maximum production of 3.35 U/g. Influence of tween-80 on lipase production was investigated, and 1.0% (v/w) of tween-80 resulted in maximum lipase production of 6.10 U/g. Thus, the statistical approach employed in this study allows for rapid identification of important medium parameters affecting the lipase production, and further statistical optimization of medium and process parameters can be explored using response surface methodology.