Quality by Design Approach for the Development and Validation of Glipizide, an Antidiabetic Drug, by RP-UPLC with Application to Formulated Forms and Urine

Table 5

Results of precision study.

Concentration injected (µg mL^−1)	Mean area ± SD	RSD^a	Mean Rt ± SD	RSD^b
Intraday precision (𝑛=7)
100	1165237 ± 2333	0.20	2.13 ± 0.006	0.26
200	2347961 ± 16728	0.71	2.13 ± 0.006	0.28
300	3482365 ± 23172	0.67	2.13 ± 0.002	0.10
Concentration injected (µg mL^−1)	Mean area ± SD	RSD^a	Mean RT ± SD	RSD^b
Interday precision (𝑛=5)
100	1161904 ± 3535	0.30	2.14 ± 0.018	0.82
200	2347961 ± 16728	0.71	2.13 ± 0.006	0.29
300	3488820 ± 7850	0.23	2.14 ± 0.020	0.91

^aRelative standard deviation based on peak area.
^bRelative standard deviation based on retention time.