Research Article

Determination of Infectious Bovine Viral Diarrhea Virus in Bovine Lung Lavages by a Combination of Virus Propagation in Cell Culture and Quantitative Real-Time PCR

Figure 1

BVDV calibration curves. (a) Calibration curve generated by RT-PCR with the cador BVDV RT-PCR Kit using virus spiked lung lavage in the range from 102.0 to 107.0 TCID50/mL. Black triangles indicate means of the Ct values of calibrator samples included in further measurements of unknown samples to proof validity of the calibration curve. (b) Calibration curve according to (a) but BVDV spiked in water in the range from 102.5 to 107.0 TCID50/mL. Shown are the mean Ct values of triplicate measurements. Raw data, including standard deviations (SD) and Ct values of the internal control, are presented in Supplementary Tables S2 and S3.
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(a)
751904.fig.001b
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