Determination of Infectious Bovine Viral Diarrhea Virus in Bovine Lung Lavages by a Combination of Virus Propagation in Cell Culture and Quantitative Real-Time PCR

Figure 2

Replication of BVDV strain NADL in MDBK cells. 7 dpi medium and cells were analyzed by RT-PCR and BVDV was quantified according to the calibration curve shown in Figure 1(a). Raw data, including standard deviations (SD) and Ct values of the internal control, are presented in Supplementary Table S4.