Migration, Integration, Survival, and Differentiation of Stem Cell-Derived Neural Progenitors in the Retina in a Pharmacological Model of Retinal Degeneration
Figure 2
Photograph of a mice retina after immunohistochemistry with three different markers. (a) Following transplantation, GFP-expression allowed histological visualization of integrated cells and extension to superficial retinal layers after 7 days following a single application with neurosphere method. The cells transplanted of the mNPC into the vitreous cavity survived, migrated, and became located into the inner retina (orange arrows). (b) mNPC expressed the indicator of presence of neuronal tissue after transplantation, anti-DAP (yellow arrows). The mNPC exhibited the neural-like morphology of living cells in the superficial retina. Donor cells occurred both as individual cells as well as occasional small clusters of cells associated with the host retina. (c) Living-cell marker, B-tubulin, enabled confirmation of donor cells in the inner retinal layers (white arrows), 40x.
