Table of Contents
ISRN Biomaterials
Volume 2013, Article ID 783873, 9 pages
Research Article

Relative Contributions of Surface Roughness and Crystalline Structure to the Biocompatibility of Titanium Nitride and Titanium Oxide Coatings Deposited by PVD and TPS Coatings

1Department of Oral Implantology, Faculty of Dentistry, Istanbul University, Capa, P.O. Box 34093, Istanbul, Turkey
2Department of Histology and Embryology, Faculty of Medicine, Istanbul University, Capa, P.O. Box 34390, Istanbul, Turkey
3Department of Metallurgical and Materials Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Maslak, P.O. Box 34469, Istanbul, Turkey
4Department of Geological Engineering, Faculty of Engineering, Istanbul University, Avcilar, P.O. Box 34320, Istanbul, Turkey

Received 25 March 2013; Accepted 19 May 2013

Academic Editors: B. F. Ali, K. Y. Choi, J. Langer, L. G. Lopes, and J. Svorec

Copyright © 2013 Sinem Yeniyol et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study was conducted to characterize titanium (Ti) metal surfaces modified by polishing, coating with titanium nitride, coating with titanium oxide, sandblasting with alumina (Al2O3) particles and coating with titanium oxide, coating with titanium plasma spray (TPS); and to evaluate the effect of surface roughness and crystalline structure on adhesion of human fetal osteoblast cells (CRL-11372) in vitro after 24 hours. Surface topography and roughness were examined by scanning electron microscopy (SEM) and a noncontacting optical profilometer, respectively. The crystalline structures of the coatings were characterized by X-ray diffraction (XRD). CRL-11372 cells were incubated at these surfaces for 24 h and were evaluated for their mean total cell counts and cell viabilities. Cell morphologies were examined qualitatively by SEM images. Glass discs served as control group (CG) for the cell culture experiments. Surfaces at the Group TPS had the highest and values. Highest mean total cell counts were found for the CG. SC (sandblasted and TiO2 coated) surfaces had shown sparsely oriented CRL-11372 cells while other surfaces and CG showed confluency. Surfaces displayed diverse crystalline structures. Crystalline structures led to different cellular adhesion responses among the groups regardless of the surface roughness values.