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ISRN Analytical Chemistry
Volume 2013 (2013), Article ID 786151, 7 pages
Research Article

Simultaneous Determination of Glycyrrhizin and 15 Flavonoids in Licorice and Blood by High Performance Liquid Chromatography with Ultraviolet Detector

1School of Life Science, Beijing Institute of Technology, Beijing 100081, China
2Institute for Food and Cosmetics Control, National Institutes for Food and Drug Control, Beijing 100050, China
3Institute of Special Wild Economic Animals and Plants, Chinese Academic of Agricultural Science, Jilin, Jilin City 132109, China

Received 8 May 2013; Accepted 11 June 2013

Academic Editors: P. Campíns-Falcó and A. Taga

Copyright © 2013 Yongqian Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Licorice is the most frequently used herb in traditional Chinese medicine (TCM) with versatile functions. It is also a popular natural dietary supplement. While the dosages is very important for there are some side effects caused by licorice. The composition of licorice, its products should be well determined thereof. A simple method for simultaneous determining sixteen compounds in vary high dynamic range of content has been established. This method based on the detection at the characteristic ultraviolet spectra of different types of compounds in licorice. Glycyrrhizin and fifteen flavonoids were well measured. All of these compounds can be precisely quantified at their characteristic wavelengths. This method has been successfully applied to the analyses of different licorices, Sini Tang decoction, and rat plasma after oral administration of Sini Tang decoction. These compounds were found to be over 3000 times in content (from 0.01  g/g to 34.5  g/g) in some samples.