Table of Contents
ISRN Tissue Engineering
Volume 2013, Article ID 825912, 8 pages
Research Article

Nanocomposites Based on PLLA and Multi Walled Carbon Nanotubes Support the Myogenic Differentiation of Murine Myoblast Cell Line

1Department of Experimental Medicine and Biochemical Science, Section of Biochemistry and Molecular Biology, University of Perugia, Via del Giochetto, 06126 Perugia, Italy
2Department of Mining-Metallurgy and Materials Science, School of Engineering, University of the Basque Country (UPV/EHU), Spain
3Materials Engineering Centre, UdR INSTM, NIPLAB, University of Perugia, Pentima Street 4, 05100 Terni, Italy
4University of Roma-La Sapienza, 00100 Roma, Italy
5Translational Cardiomyology Lab, Department of Development and Regeneration, SCIL, University of Leuven, 3000 Leuven, Belgium
6Department of Public Health, Neuroscience, Experimental and Forensic Medicine and Center for Tissue Engineering (CIT), University of Pavia, 27100 Pavia, Italy
7Institute of Polymer Science and Technology (ICTP), CSIC, Calle de Juan de la Cierva 3, 28006 Madrid, Spain

Received 18 April 2013; Accepted 26 May 2013

Academic Editors: C. Haasper, J. Mansbridge, and Y.-J. Ren

Copyright © 2013 Simona Montesano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We explored the effect of poly(L-lactic acid) (PLLA) containing various percentages (0.1, 0.5, 1, and 3 wt.%) of multi walled carbon nanotubes (MWCNTs) on the myogenic differentiation of C2C12 murine myoblast progenitor cells. We showed that all PLLA/MWCNTs nanocomposite materials support the myotubes formation more efficiently than neat PLLA as indicated by the high expression of the most significant myogenic markers: MyoD, Myosin Heavy Chain, dimension of myofibres, and fusion myogenic index. Interestingly, we note that both MyoD and myogenic fusion index levels were in the order 0.1 MWCNTs = 0.5 MWCNTs > 1 MWCNTs > 3 MWCNTs > neat PLLA, suggesting that the amount of MWCNTs influenced the cell differentiation.