Proteomics Characterization of the Secretome from Rat Pancreatic Stellate Cells with ATP-Binding Cassette Transporters (ABCG2) and NCAM Phenotype

Table 1

List of all the primers used in this study.

Name	Sequence	bp	F/R	T annealing	GenBank accession numbers
Calreticulin	GGGCCCCTGATACCAAATGTC	467	FW	57°C	XM_001067664
	CTCTCGCCGCTGCCTTCC		RV
Wnt5b	GCGCTGCTGGTGTGGTGAATG	415	FW	60°C	XM_342747
	CCGGGCTGGGCTGGTTGA		RV
Calumenin	AGCTCCGGGGAAAACTCAG	501	FW	60°C	NM_022535
	CTCCGCTCATCTCTAACCATAATC		RV
SPARC	AAACCCCTGCCAGAACCATCATTG	415	FW	60°C	NM_012656
	CTCCAGGCGCTTCTCGTTCTCGTG		RV
DIXDC1	GGGCAAATATGGATAAGGATGAGC	435	FW	57°C	NM_001037654
	GTGACCGCCCCCGAACC		RV
Neuromodulin (GAP 43)	CTCCCGTTGCTGATGGTGTGG	453	FW	60.5°C	NM_017195
	GGTCAGCCTCGGGGTCTTCTTTAC		RV
Synaptotagmin V	CAGGGCAGAACACGCACGCACATA	524	FW	59°C	BC_092198
	CTCCGGCCCAGCTCCTTCACTTCC		RV
Transcobalamin 2	TGGGCCAGCGTCTCTTACCTT	508	FW	60.5°C	NM_022534
	CCGGCCATGGCTTCTGTGTC		RV
PEDF (serpin F1)	GGAGCCCGTAGTGGAGGAGGATGA	417	FW	60°C	BC_078686
	ATGCGAGGGTTGCCAGTGAGGATT		RV
AHNAK	GTCGGCTCCTCAAGTCTCCATACC	482	FW	57°C	XR_005457
	TCCCCGACACTTTCACACCATCT		RV
DHRS4	AGGCGGCTCGGTGGTGATT	441	FW	58°C	NM_153315
	TGGCGGTGGCAAGGAAGG		RV
Glypican 1 variant	GCTGCTGCTGCCCGATGACTAT	489	FW	61°C	NM_030828
	CCGCAGCCCTGGATGACCTTA		RV
RAB15	GCCGGGCCGCTTCCTTCT	404	FW	61°C	NM_198749
	GCGCTCGCTGCTAATGTCGTA		RV
ARHGEF2	AGACCCGGGAAAAGGAGAAGATGA	445	FW	58°C	NM_001012079
	TGACGCAGCCCCAGAGGAGAC		RV
Islet cell autoantigen 1-like	TCGGTTTGCTCAAGATAAGTCAGT	404	FW	54.5°C	NM_030844
	TGGCCCGATGTCTAAAAGTCTCTA		RV
Leukemia inhibitory factor	AGGTCTTGGCCGCAGGGATTG	508	FW	58°C	NM_022196
	TCGGGGACACAGGGCACAT		RV
ERABP	CATGGTGGTCGAGCTGAAAGAGAA	448	FW	55°C	NM_024136
	GTAGGGAAGGAGAGGCCAGGTAGG		RV
RAB14	TCGGGGATATGGGAGTAGGAA	541	FW	54.5°C	NM_053589
	GGGGCTGAGGGTTTGTGTTGTA		RV
APG16 autophagy 16-like	CGGCCACAGCGGGAAAGTT	542	FW	58°C	XM_001067061
	CCCACGCCACCGCATTGATAGAAG		RV
MMP2	GCTGATACTGACACTGGTACTG	217	FW	60°C	NM_031054
	CAATCTTTTCTGGGAGCTC		RV
Collagen I	GCCACCTCAAGAGAAGTC	440	FW	58.5°C	BC133728.1
	ATAGCGACATCGGCAGGATCG		RV
Vimentin	GCCAGCAGTATGAAAGTGTG	496	FW	60°C	NM_031140
	AGTGGGTGTCAACCAGAGGAA		RV
Desmin	AGACTTGACTCAGGCAGCCAAT	384	FW	60°C	NM_022531
	CGGAAGTTGAGAGCAGAGAAGG		RV
GFAP	TGGCCACCAGTAACATGCA	538	FW	60°C	NM_017009
	GACTCCTTAATGACCTCGCCAT		RV
TBP	ACCCTTCACCAATGACTCCTATG	190	FW	60°C	NM_001004198
	ATGATGACTGCAGCAAATCGC		RV
PDX1	GAGCCCAGCCGCGTTCATCT	318	FW	60°C	NM_022852
	CCCCGCTCGTTGTCCCGCTACTA		RV
CK19	ACAGCCAGTACTTCAAGACC	690	FW	57°C	AY_464140
	CTGTGTCAGCACGCACGTTA		RV
RTGFB I	ACCGCGTGCCAAATGAAGAGGAT	464	FW	55°C	L26110.1
	TGCCGTGGACAGAGCGAGTTTGAT		RV
RTGFB II	TGGCCGCTGCACATCGTCCTG	366	FW	55°C	AF474028.1
	CTCGCCCGCCCTTTTCTTTTCCTT		RV
RTGFB III	AGCAGCGCGGCCCACAGCATC	531	FW	55°C	NM_017256.1
	GAAGGGGGCATCCAGGGCGAGACT		RV
TBP real time	TTCGTGCCAGAAATGCTGAA		FW		NM_001004198
	GTTCGTGGCTCTCTTATTCTCATG		RV
PDX1 real time	CCGCGTTCATCTCCCTTTC		FW		NM_022852
	CTCCTGCCCACTGGCTTTT		RV
CK19 real time	AAGGTCCAGGACCTTGAGATTG		FW		AY_464140