Proteomics Characterization of the Secretome from Rat Pancreatic Stellate Cells with ATP-Binding Cassette Transporters (ABCG2) and NCAM Phenotype

Table 2

List of the proteins obtained in the secretome of PaSCs-ABCG2(+).

Protein number	Protein name	UniProt ID	pI (T)	MW (T)	pI (E)	MW (E)	n.pep	% Cov	Secretion
Proteolysis and related proteins
1	Procollagen C-endopeptidase enhancer 1	O08628	8,2	48000	7,1	55793	8	18	1
1	Procollagen C-endopeptidase enhancer 1	O08628	8,2	48000	7,6	55793	7	18	1
1	Procollagen C-endopeptidase enhancer 1	O08628	8,2	48000	6,7	56509	11	33	1
1	Procollagen C-endopeptidase enhancer 1	O08628	8,2	48000	6,2	57108	10	28	1
2	72 kDa type IV collagenase (MMP2)	P33436	5,1	62000	5,0	68834	16	26	1
3	Alpha-2 antiplasmin	Q80ZA3	6	46000	5,5	52395	10	28	1
3	Alpha-2 antiplasmin	Q80ZA3	6	46000	5,4	53373	9	26	1
4	Isoform 4 of ubiquitin carboxyl-terminal hydrolase 15	Q8R5H1-4	5,4	26000	5,3	31776	8	38	2
5	Adult male cDNA proteasome regulatory particle	Q8C1T2	4,9	28000	4,9	31776	5	22	3
6	Cathepsin B.	Q6IN22	5,5	38000	5,1	42629	6	23	1
7	ATPase 3 (Fragment)	Q6PW16	6,2	14000	7,1	27988	7	57	2
Structural and cytoskeleton proteins
8	Glial fibrillary acidic protein (fragment)	Q866S9	5,2	48000	4,7	44342	11	24	2
8	Glial fibrillary acidic protein (fragment)	P03995	5,4	50000	5,0	31776	9	21	2
9	Testis cDNA, similar to human desmin	Q4R7K6	5,1	24000	4,7	24070	12	38	2
10	Vimentin	P31000	5,1	54000	4,5	23085	15	22	2
10	Vimentin	P31000	5,1	54000	5,1	22777	14	23	2
10	Vimentin	P31000	5,1	54000	4,6	21138	17	28	2
10	Vimentin	P31000	5,1	54000	4,6	20825	13	22	2
11	Tropomyosin-1	Q60527	4,6	33000	4,6	40365	16	51	3
11	Tropomyosin alpha isoform	Q923Z2	4,7	33000	4,6	36876	8	24	3
11	Tropomyosin isoform 8 of P04692	P04692-8	4,8	29000	4,9	37590	7	24	3
11	Tropomyosin isoform 4 of P04692	P04692-4	4,8	29000	5,4	42564	10	38	3
12	Alpha-tropomyosin 3	Q63607	4,7	33000	9,3	49605	11	29	2
13	Tropomyosin 3, gamma	Q8K0Z5	4,7	33000	4,8	27739	10	32	3
14	Tropomyosin isoform 6	Q63610	4,8	29000	4,7	32061	14	46	3
14	Tropomyosin isoform 6	Q63610	4,8	29000	4,8	32157	8	36	3
15	Tropomyosin alpha-4 chain	P09495	4,7	28000	4,6	32471	15	43	3
16	Lamin-A	P48679	6,5	74000	5,2	42694	13	21	3
16	LMNA protein	Q8N519	6	53000	5,5	38527	10	19	3
17	Lamin-B2	P21619	5,4	67000	5,6	59799	12	18	3
18	Transgelin.	P31232	8,9	22000	9,6	24360	7	40	2
19	Myosin light polypeptide 6	Q64119	4,5	17000	4,3	19735	5	39	1
Associated with cell growth, development, and differentiation
20	Neuromodulin	Q6S9D9	4,7	25000	4,5	23714	9	22	3
21	Pigment epithelium-derived factor	P97298	6,5	44000	5,5	52395	8	20	1
21	Pigment epithelium-derived factor	P97298	6,5	44000	5,4	51521	6	14	1
22	Protein Wnt-5b	Q9H1J7	8,8	39000	5,7	23432	8	20	1
23	DIXDC1 protein (fragment)	Q8IVY4	6	41000	6,0	33333	9	24	3
24	AHNAK 1 (fragment)	Q38PG1	5,2	59000	5,2	38623	14	22	3
25	SPARC	Q6GSZ4	4,8	34000	4,7	44342	15	38	1
26	Leukemia inhibitory factor	P17777	9	20000	6,0	23432	4	30	1
Chaperones
27	Calreticulin	P18418	4,3	46000	4,3	53430	14	38	1
28	Heat shock protein 8	Q4FZY7	5,4	71000	5,4	69248	26	51	3
29	78 kDa glucose-regulated protein	P06761	5	70000	5,0	68834	13	25	1
30	Serpinh1 protein	Q5RJR9	8,9	47000	9,3	49605	17	40	1
31	Calumenin	O35783	4,4	35000	4,3	49231	5	24	1
Metabolism
32	Phosphoglycerate kinase 1	P16617	7,5	44000	8,0	46687	14	41	3
33	Enolase 1, alpha	Q5EB49	6,2	47000	6,0	53471	10	28	2
34	Fructose-bisphosphate aldolase A	P05065	8,4	39000	8,8	42888	12	29	3
35	Isoform M2 of Pyruvate kinase	P11980-2	7,4	58000	7,4	61394	23	52	3
36	Carbonyl reductase [NADPH] 1	P47727	8,2	30000	6,7	27573	9	32	2
37	Adult male cDNA, similar to GTPasa	Q3UH20	7	43000	4,6	28837	12	20	3
Transport proteins
38	Transcobalamin-2	Q9R0D6	7,8	46000	8,0	46687	11	25	1
39	Ferritin light chain 1	Q6P7T1	6	21000	6,0	20981	7	53	3
40	Syntaxin-binding protein 3	Q60770	8,3	68000	6,0	64613	16	24	3
41	Synaptotagmin 5	Q56A28	9,4	43000	9,1	43084	8	20	1
42	AP-4 complex subunit sigma-1	Q9Y587	5,1	17000	3,7	18044	4	36	3
GTPase-related proteins
43	Ras-related protein Rab-14	Q91V41	5,9	24000	6,0	23432	7	39	3
44	RAB15, member RAS oncogene family	Q504L6	5,4	24000	4,4	19298	6	29	1
45	Rho/Rac guanine nucleotide exchange fact	Q92974	8,7	101000	7,0	89066	6	8	3
Immune response
46	MHC class I antigen	Q9MXM3	5,5	40000	5,9	30475	10	38	1
47	Lysozyme C	P61626	9,3	15000	8,5	18234	7	39	1
48	Cutaneous T-cell lymphoma-associated antigen	Q8IX95	4,9	18000	6,0	38747	5	48	2
49	Recombination-activating gene 1	Q5W9V9	6,4	38000	4,6	37876	11	41	2
50	Islet cell autoantigen 1-like protein	Q6RUG5	5,2	49000	5,4	59799	8	24	3
51	APG16 autophagy 16-like	Q3TDQ5	5,9	66000	7,0	61394	8	18	3
Extracellular matrix and related proteins
52	Alpha-1 type I procollagen (fragment)	Q60785	5,7	35000	5,2	34660	11	42	1
53	Col1a1 protein (fragment)	Q99LL6	5,9	59000	5,7	51246	7	20	1
53	Col1a1 protein (fragment)	Q99LL6	5,9	59000	5,6	51626	12	30	1
54	Col1a2 protein (fragment)	Q91VL4	7,1	57000	6,0	32431	7	15	1
55	procollagen, type III, alpha 1	Q8BJU6	5,9	36000	5,3	33602	10	36	1
56	Collagen alpha-1 (III) chain	P13941	9,4	94000	7,6	174792	15	20	1
57	Collagen alpha 1 (V) (fragment)	O70603	4,8	22000	4,4	36028	7	52	1
58	Glypican 1 variant (fragment)	Q59GI7	6,5	36000	5,6	33085	6	22	1
59	Perlecan	Q05793	5,9	17800	6,2	23432	8	11	1
Retinol-related proteins
60	Dehydrogenase/reductase SDR family member	Q9BTZ2	7,7	28000	8,0	36876	4	11	3
61	Epididymal retinoic acid-binding protein	P06911	5,5	18000	5,3	23432	4	37	1

Secretion is indicated with (1) if the protein had a predicted signal peptide, (2) if it had a score >0,5 in neural network and therefore are nonclassically secreted, or (3) if they are not predicted as secreted. Proteins are listed with the accession number of Swiss-Prot and grouped in clusters of families and functions. (T) MW and pI as determined by UniProt database; (E) experimentally found in our 2D gels. % Cov: % of the sequence coverage by peptide mass fingerprinting using Aldente software, n.pep.: number of peptide masses matched.