Figure 3: The effect of 2-AP on protein expression in the aorta. Distal regions of aortas from control and 2-AP treated apoE −/− mice were minced and homogenized. Protein levels in the aorta extract were determined with western blot analysis using antibodies against eIF-2α and phosphorylated eIF-2α (p-eIF2α), GRP78, LAL (liposomal acid lipase), and β-actin. The level of p-eIF2α was expressed relative to the eIF-2α immunoblot intensity. The levels of the other proteins were expressed relative to β-actin. Data are expressed as the mean ± SEM of three independent experiments. * as compared to untreated control mice.