Use of a Multiplex PCR for the Detection of Toxin-Encoding Genes netB and tpeL in Strains of Clostridium perfringens

Table 2

Specifications of primers used in a multiplex PCR for the detection of netB and tpeL.

Primer ID	Sequence	Target gene	Product length
netB5F	CGCTTCACATAAAGGTTGGAAGGC	netB	316
netB5R	TCCAGCACCAGCAGTTTTTCCT
AKP80*	ATATAGAGTCAAGCAGTGGAG	tpeL	466
AKP81*	GGAATACCACTTGATATACCTG

∗Primers previously published by Keyburn et al., 2010 [7].