Generation of Constitutive Active ERK Mutants as Tools for Cancer Research in Zebrafish

Table 4

Cluster 6 peptides are increasingly phosphorylated by FGF8 or ERK2*** but not ERK2WT samples. ANOVA and Dunnett’s test are performed with a biological triplicate.

Peptide	Fold change (from mean 2log)				𝑃 values versus  WT			𝑃 values versus ERK2_WT
	WT	ERK2WT	ERK2***	FGF8	ERK2WT	ERK2***	FGF8	ERK2***	FGF8
RBL2_655_667	1.00	0.93	1.11	1.31	0.772	0.671	0.205	0.482	0.135
PRKDC_2618_2630	1.00	0.98	1.17	1.63	0.734	0.618	0.267	0.412	0.165
PP2AB_297_309	1.00	0.88	1.11	1.27	0.270	0.307	0.510	0.058	0.104
MBP_222_234	1.00	0.94	1.19	1.05	0.697	0.055	0.591	0.032^†	0.367
NEK3_158_170	1.00	1.21	1.27	1.50	0.516	0.093	0.031^†	0.240	0.080
KCC2G_278_289	1.00	1.02	1.28	1.43	0.537	0.099	0.012^†	0.040^†	0.006^†
CGHB_109_121	1.00	0.97	1.29	1.20	0.446	0.064	0.143	0.022^†	0.047^†
CDC2_154_169	1.00	0.95	1.11	1.15	0.982	0.041^†	0.037^†	0.042^†	0.038^†

^†𝑃 values < 0.05.