Antiallergic and Antiarthritic Effects of Stem Bark Extract of Glyphaea brevis (Spreng) Monachino (Tiliaceae) in Murine Models
Figure 5
Effect of G. brevis extract (GBE) on adjuvant-induced arthritis. Sprague-Dawley rats (200–250 g) were injected intraplantar with 100 μL of CFA or IFA into the right hind paw. Foot volume was measured by water displacement plethysmography daily for 28 days. The oedema component of inflammation was monitored as the percentage change in paw volume (a, c, and e) and the total oedema induced during the acute and polyarthritis phases calculated as area under the time course curves, AUC (b, d, and f). In the preventive protocol (top and middle panels), drug vehicle, diclofenac 6 mg kg−1, or GBE 30, 100, and 300 mg kg−1 were given orally 1 h before the induction of the arthritis and daily for 28 days while, in the curative protocol (bottom panel), treatment commenced 10 days after arthritis induction. Drug effects were evaluated by comparing the maximal and total oedema responses attained during 28 days in drug-treated groups with the corresponding values attained in drug-vehicle-treated inflamed control groups. Data is presented as mean ± s.e.m. () ***, **, ** when compared with vehicle-treated control group. Arrow indicates point of extract administration in the therapeutic protocol.
