Lesion-Induced Alterations in Astrocyte Glutamate Transporter Expression and Function in the Hippocampus
Figure 5
GLAST and GLT-1 immunoreactivity after mechanical injury. (a) Fluorescence images of immunohistochemical stainings detecting the glutamate transporters GLAST (left), GFAP (center) as counterstaining, and the corresponding merged image (GFAP: green, GLAST: magenta) in control (top row) and lesioned slice cultures (bottom row). (b) GLT-1 (left), GFAP (center) double labeling of a control (top row) and a mechanically lesioned slice (bottom row). The corresponding merge (GFAP: green, GLT-1: magenta) is found on the right. Note that after lesion, glutamate transporter immunoreactive clusters are accumulated in the direct vicinity of the lesion site (arrowheads). GFAP, glial fibrillary acidic protein; GLAST, glutamate aspartate transporter; GLT-1, glutamate-transporter-1; StrO, stratum oriens; StrP, stratum pyramidale; StrR, stratum radiatum; StrLM, stratum lacunosum-moleculare; and scale bars: (a) 20 μm, (b) 40 μm.
