Table of Contents
ISRN Spectroscopy
Volume 2013, Article ID 912376, 5 pages
Research Article

A New Analytical Q-Absorbance Ratio Method Development and Validation for Simultaneous Estimation of Lamivudine and Isoniazid

Department of Pharmaceutics, Indian Institute of Technology (Banaras Hindu University), Varanasi Uttar Pradesh 221005, India

Received 27 August 2013; Accepted 17 November 2013

Academic Editors: D. Chattopadhyay, A. A. Ensafi, B. Kiran, R. Schneider, and A. Toncelli

Copyright © 2013 Gitu Pandey and Brahmeshwar Mishra. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A new UV spectrophotometric absorption ratio method was developed and validated for the simultaneous estimation of lamivudine and isoniazid. The method involved Q-absorption ratio analysis using two wavelengths, with one being the of lamivudine (272 nm, ) and the other being the isoabsorptive point of both drugs (246 nm, ). Beer’s law was obeyed in the concentration range between 5 and 30 µg/mL for both lamivudine and isoniazid. The results of analysis have been validated statistically and by recovery studies as per ICH guidelines. The accuracy ranged between 99.65 and 101.91% and Sandell’s sensitivity ranged between 0.0229 and 0.0347 µg/cm2. The method was found to be simple, precise, reproducible, rapid, and economical. Hence, it could be used in the analysis of laboratory samples and marketed formulations containing these two drugs in the future.