Challenges in Enzymatic Route of Mannitol Production

Table 2

Recombinant strains of E. coli where mdh genes from different microbes have been cloned for mannitol production. (source from BRENDA database).

Cloned/Commentary	Organism	Literature
MtDH is expressed in Escherichia coli BL21 (DE3)	Lactobacillus reuteri	[46]
Fusion of six His codons to the 3′ end of the mdh gene and expression in Escherichia coli M15. The enzyme shares significant sequence similarity with the medium-chain dehydrogenase/reductase protein family	Leuconostoc mesenteroides	[52]
MDH expression in Escherichia coli, affecting strong catalytic activity of NADH-dependent reduction of D-fructose to D-mannitol in cell extracts of the recombinant Escherichia coli strain	Leuconostoc pseudomesenteroides	[53]
Gene mdh, high level expression in Bacillus	Leuconostoc pseudomesenteroides	[12]
MDH gene subcloned into vector pDEST110 and overexpressed in different strains of Escherichia coli (BL21 (DE3) plysS, JM109, Origami(DE3) or M15)	Pseudomonas fluorescens	[54]
MDH expressed in Escherichia coli BL21 (DE3) cells; the TM0298 gene subcloned into the NdeI and XhoI sites of pET24a(+) to yield plasmid pTmMtDH, from which MtDH is expressed with a C-terminal His6-tag in Escherichia coli BL21 (DE3)	Thermotoga maritime	[46]
Gene mtdh, expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)	Thermotoga maritime	[46]