Table of Contents
ISRN Microbiology
Volume 2013, Article ID 918921, 5 pages
http://dx.doi.org/10.1155/2013/918921
Research Article

Antibiotic Resistance and Biofilm Production in Staphylococcus epidermidis Strains, Isolated from a Tertiary Care Hospital in Mexico City

1Department of Public Health, Faculty of Medicine, National Autonomous University of Mexico, 04510 Mexico City, DF, Mexico
2Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico, 04510 Mexico City, DF, Mexico

Received 28 September 2012; Accepted 17 October 2012

Academic Editors: S. H. Flint, J. Kreth, and P. Zunino

Copyright © 2013 Roberto Cabrera-Contreras et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Staphylococcus epidermidis strains isolated from nosocomial infections represent a serious problem worldwide. In various Mexican states several reports have shown isolates from hospitals with antibiotic resistance to methicillin. In Mexico City, there is scarce information on staphylococcal infections in hospitals. Here, our research findings are shown in a four-year period study (2006–2010) for Staphylococcus epidermidis strains. Susceptibility and/or resistance to antibiotics in SE strains were assessed by phenotypic and molecular methods as mecA gene by PCR, as well as the correlation with biofilm production for these isolates and the relationship to the infection site. Out of a total of 161 (66%) negative biofilm SE strains, just 103 (64%) SE strains were confirmed as MRSE by PCR to mecA gene. From 84 (34%) positive biofilm SE strains, 76 (91%) were confirmed as MRSE by PCR to mecA gene. Higher percentages of resistance to antibiotics and higher number of resistance markers were found in biofilm-forming clinical strains (9 to 14) than non-biofilm-forming SE strains (3 to 8). These research findings represent a guide to establish infection control programs for this hospital.