Changes in 𝜸-Tubulin Protein Distribution in Zebrafish (Danio rerio) Oocytes and the Early Cleavage-Stage Embryo

Figure 3

During cleavage, 𝛾-tubulin is found in various structures including punctate foci (P) and arc-shaped/rings (A/R) revealed by GTU-88 staining as well as diffuse presumably soluble, 𝛾-tubulin in the cytoplasm of blastomeres. (a)–(c) Serial optical sections through a 32-cell stage embryo revealing the extent of the A/R structures (arrows) surrounding interphase nuclei (n); DAPI (red), GTU-88 (green), bar equals 100 μm. (d) Early blastula showing both punctate foci (P) and arc-shaped/ring (A/R) structures stained with GTU-88, presumably associated with asynchronous blastomeres at different stages of the cell cycle; bar equals 100 μm. (e) 2-cell stage with punctate foci (P) of 𝛾-tubulin (green) at the spindle poles of metaphase blastomeres, nuclei/chromosomes (n) stained with DAPI (red); bar equals 100 μm. (f) Early blastula with predominately mitotic blastomeres showing GTU-88 staining (green) in punctate foci (P) at the spindle poles of DAPI-stained (red) mitotic figures in metaphase (m) and anaphase (a); bar equals 100 μm.