Critical Analysis of Strand-Biased Somatic Mutation Signatures in TP53 versus Ig Genes, in Genome-Wide Data and the Etiology of Cancer
Table 1
Typical patterns of somatic point mutations observed at rearranged immunoglobulin variable region loci in mice.
(a) Studies where PCR recombinant artefacts in DNA sequence data absent or minimized
Original base
Mutant base
A
T
C
G
Total
A
—
10.6 (1.2)
6.3 (0.9)
14.6 (0.7)
31.6 (1.7)
T
3.1 (0.6)
—
5.3 (1.1)
2.6 (0.6)
11.0 (1.3)
C
4.3 (0.8)
13.4 (1.3)
—
3.6 (0.7)
21.3 (1.3)
G
20.1 (1.9)
7.2 (1.4)
8.7 (0.7)
—
36.1 (2.5)
(b) Studies where PCR recombinant artefacts in DNA sequence data were not minimized
Original base
Mutant base
A
T
C
G
Total
A
—
9.9 (0.5)
7.3 (0.5)
16.5 (0.5)
33.7 (0.7)
T
5.4 (0.3)
—
9.0 (0.5)
3.6 (0.3)
18.0 (0.6)
C
3.7 (0.3)
15.0 (0.8)
—
5.9 (0.6)
24.6 (1.0)
G
14.4 (0.6)
4.2 (0.4)
6.5 (0.7)
—
24.4 (0.7)
Data taken from Table 1 in [3]. Each entry is the mean percentage and standard error of the mean (shown in brackets) for each type of mutation rounded to one decimal place. All mutations scored from the coding or nontranscribed strand (NTS). Data in all studies were corrected for the base composition of the V region or 3′ flanks assayed for mutations. In (a), data averaged over twelve studies (); in (b), data averaged over twenty studies (). Note that one study by Winter et al. [9] inadvertently left out of the survey (see Supplementary data in [3] in Supplementary material available online on http://dx.doi.org/10.1155/2013/921418) but its omission does not change the patterns documented in (b).
