Table of Contents
ISRN Virology
Volume 2013 (2013), Article ID 924057, 7 pages
http://dx.doi.org/10.5402/2013/924057
Research Article

Immunogenicity of NS4b Dengue 3 Virus Mimotope Presented to the Immune System as Multiple Antigen Peptide System

1Finlay Institute, calle 27, No. 19805, La Lisa, AP 16017, cod 11600 Havana, Cuba
2Department of Virology, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Pedro Kourí Tropical Medicine Institute (IPK), Autopista Novia del Mediodía Km 6, Apdo 601, Marianao 13, Havana, Cuba
3Center for Genetic Engineering and Biotechnology (CIGB), Avenue 31, P.O. Box 6162, 10600 Habana, Cuba
4Institute of Infection, Immunity and Inflammation, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK

Received 27 June 2013; Accepted 17 July 2013

Academic Editors: H. Imataka, P. Luksamijarulkul, M. Magnani, and S. Pöhlmann

Copyright © 2013 Nevis Amin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The availability of random peptide libraries displayed on bacteriophage (RPL) has provided a powerful tool for selecting sequences that mimic binding properties of natural antigen epitopes (mimotopes). These mimotopes can be used for vaccine design, drug development, and diagnostic assays. Several mimotopes have been shown to induce production of antibodies against the natural antigen. We have previously identified four dengue virus mimotopes from a phage-displayed peptide library using antidengue 3 human sera. Three of them showed similarity in their amino acid sequences with the NS4b proteins of dengue. Few studies have examined the role of NS4b proteins in the antibody response to dengue virus infection. A multiple antigen peptide (MAP) system was chemically synthesized containing this mimotope (NS4b MAP), and BALB/c mice were immunized to evaluate its immunogenicity. Antipeptide responses were induced and recognised DENV-3 infected cells as determined by immunofluorescence. The high levels of the IgG2a subtype against NS4bMAP suggest the induction of a Th1-like response. Our findings suggest that the NS4b mimotope might be a useful tool for the development of multiepitope diagnostic assays, dengue virus vaccine design, and pathogenesis studies.