Colombian Anopheles triannulatus (Diptera: Culicidae) Naturally Infected with Plasmodium spp.

Table 3

Natural infection on Anopheles triannulatus from Northwestern and Southeastern Colombia.

Region/locality/collection site	No. of; An. triannulatus analyzed	Plasmodium species^a: infection rates^b  (95% CI)	ELISA test		PCR
			First	Second	Nested	Cytb
				(confirmation)
NW
El Bagre-BAG, La Capilla	82	PvVK247: 1.51 (0.04–8.15)	Positive	Negative	Positive	Negative
Puerto Libertador-PLT, Juan José	83	Pf: 1.20 (0.03–6.53)	Negative	Negative	Positive	Positive
SE
Puerto Nariño (PNA), Puerto Rico	83	Pf: 1.20 (0.03–6.53)	Positive	Negative	Positive*	Negative
Tarapaca (TAR), Ventura	118	Pf: 0.85 (0.02–4.63)	Positive	Negative	Positive*	Negative

The table shows only results for those localities where naturally infected An. triannulatus were detected. aPlasmodium species: PvVK247-P. vivax VK247, PvVK210-P. vivax VK210, Pf: P. falciparum.bNumber of positive An. triannulatus (np) per number of total analyzed (nt) per 100, determined for each locality, (IR = (np/nt) × 100). *Positive result only in the nested PCR that detects Plasmodium genus, negative in the nested PCR that detects Plasmodium species. Due to the limitations on the available tests for detecting naturally infected anopheline mosquitoes (see discussion), positive specimens were determined as those given a positive result in at least two of the tests.