Production of Alkaline Protease by Solvent-Tolerant Alkaliphilic Bacillus circulans MTCC 7942 Isolated from Hydrocarbon Contaminated Habitat: Process Parameters Optimization

Table 2

Effect of nitrogen sources on protease production by B. circulans MTCC 7942.

Nitrogen supplementation (1%)^#	Protease activity; (U/mL ± SD)	Specific activity; (U/mg ± SD)
Feather meal	69.5±6.9	222.6±21
Bovine serum albumin	216.5±18.4	635.9±55
Beef extract	300.6±12.0	905.7±41
Casein	320.7±6.0	884.2±114
Casamino acids	329.1±13.3	966±31
Yeast extract	330.1±23.8	875.8±163
Gelatin	339.2±10.0	1063.0±39
Peptone	357.0±10.5	1007.0±75
Soybean meal	397.0±7.6	1164.0±33

#Basal medium containing (g/L): starch, 20; KH₂PO₄, 1; MgSO₄·7H₂O, 0.05; CaCl₂, 1; Na₂CO₃, 8 and cultural parameters (pH 10.0) at 37°C and 100 rpm.