Table of Contents
ISRN Pharmacology
Volume 2013 (2013), Article ID 949871, 9 pages
http://dx.doi.org/10.1155/2013/949871
Research Article

Investigating Apoptotic Effects of Methanolic Extract of Dorema glabrum Seed on WEHI-164 Cells

1Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran
2Tabriz University of Medical Sciences, International Branch (Aras), Jolfa 5441643177, Iran
3Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran
4Immunology Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, Iran

Received 16 April 2013; Accepted 11 June 2013

Academic Editors: H. Cerecetto, G. Edwards, and G. Gervasini

Copyright © 2013 Maryam Bannazadeh Amirkhiz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We aimed to investigate the apoptotic effects of the methanolic extract of Dorema glabrum seed on WEHI-164, cancerous cells in comparison with L929, normal cells and compared them with the cytotoxic effects of Taxol. So, MTT test and DNA fragmentation assay were performed on cultured and treated cells. Also electrophoresis which was followed by immunoblotting was done to survey the production of Caspase-3 and Bcl2 proteins, and to inquire into their relative genes expression, RT-PCR was used. According to our findings, the methanolic extract of Dorema glabrum seed can alter cells morphology as they shrink and take a spherical shape and lose their attachment too. So, the plant extract inhibits cell growth albeit in a time- and dose-dependent manner and results in degradation of chromosomal DNA. Induction of apoptosis by the plant extract was proved by the reduction of pro-Caspase-3 and Bcl2 proteins and increase in Caspase-3 gene expression and decrease in that of bcl2 too. Our data well established the antiproliferative effect of methanolic extract of Dorema glabrum seed and clearly showed that the plant extract can induce apoptosis and not necrosis in vitro. These results demonstrated that Dorema glabrum seed might be a novel and attractive therapeutic candidate for tumor treatment.