Mutation Screening of Elongation Factor 2 in Shwachman-Diamond Syndrome Patients Lacking Mutations in the SBDS Gene

Table 1

Primers sequences and amplicon size of the 11 fragments used for the analysis of the EEF2 gene.

Exon	Forward primer	Reverse primer	Amplicon length
1	cctataaaaagctgagtgttgacgtc	caccagcgaggcagggt	142
2	gtgccatgctgtgttcctggaaat	tcgctggactgaacctcactcatt	425
3-4	atgaatcttgggaccaagtcggtg	ctgtcacccaacattcctggcaaa	872
5-6	gtctactgaagaaagcctgcgtct	tccagatcttaagagaggagccctga	765
7	tgggctttcttgtttcttggtggg	tgtcagagcatccggaaacagca	211
8-9	tggcttcaagagaggaagcgtgta	atgctccttacttctagctcccga	649
10	tgaagaggatgtttcctgacagcc	agtctccatttacagccacagcca	582
11	ttcccagcaccttccctgaaatct	cacatgcctgtggaagactgcaaa	396
12	aggatgcgtctgtgtgtaaggtca	atgaggtccctctagagcctggaa	481
13-14	acttggcaggtggagggcaa	tcaggacgcctcctttaacacctt	563
15	tgagctcctgacaggactttccttctgccct	ccctgcgaagacaccgtgaaa	803