Research Article
Enhanced Cellulase Production from Bacillus subtilis by Optimizing Physical Parameters for Bioethanol Production
Table 1
CCD showing experimental and regression model predicted CMCase activity (U/mL) and cell growth (g/L).
| Run no. | pH | Temp (°C) | Agitation (rpm) | CMCase activity (U/mL) | Cell growth (g/L) | Measured | Predicted | Measured | Predicted |
| 1 | 0 (7.0) | 0 (37) | 0 (180) | |
0.437
| | 3.18 | 2 | 1 (7.6) | −1 (33) | 1 (215) | |
0.335
| | 2.39 | 3 | −1 (6.4) | 1 (41) | −1 (144) | |
0.512
| | 1.85 | 4 | α (8.0) | 0 (37) | 0 (180) | |
0.503
| | 2.53 | 5 | 0 (7.0) | 0 (37) | −α (120) | |
0.490
| | 2.14 | 6 | (5.9) | 0 (37) | 0 (180) | |
0.360
| | 2.10 | 7 | 0 (7.0) | 0 (37) | 0 (180) | |
0.437
| | 3.18 | 8 | 0 (7.0) | 0 (37) | 0 (180) | |
0.437
| | 3.18 | 9 | 0 (7.0) | 0 (37) | 0 (180) | |
0.437
| | 3.18 | 10 | 1 (7.6) | 1 (41) | −1 (144) | |
0.538
| | 2.06 | 11 | 1 (7.6) | 1 (41) | 1 (215) | |
0.374
| | 2.93 | 12 | 0 (7.0) | 0 (37) | 0 (180) | |
0.437
| | 3.18 | 13 | −1 (6.4) | −1 (33) | −1 (144) | |
0.202
| | 1.75 | 14 | 0 (7.0) | 0 (37) | 0 (180) | |
0.437
| | 3.18 | 15 | 1 (7.6) | −1 (33) | −1 (144) | |
0.325
| | 2.01 | 16 | −1 (6.4) | 1 (41) | 1 (215) | |
0.327
| | 2.67 | 17 | 0 (7.0) | 0 (37) | α (240) | |
0.344
| | 3.15 | 18 | 0 (7.0) | α (44) | 0 (180) | |
0.365
| | 2.13 | 19 | 0 (7.0) | −α (30) | 0 (180) | |
0.071
| | 1.58 | 20 | −1 (6.4) | −1 (33) | 1 (215) | |
0.192
| | 2.08 |
|
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Values are mean ± SE ().
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