International Scholarly Research Notices / 2013 / Article / Tab 1

Research Article

Enhanced Cellulase Production from Bacillus subtilis by Optimizing Physical Parameters for Bioethanol Production

Table 1

CCD showing experimental and regression model predicted CMCase activity (U/mL) and cell growth (g/L).

Run no.pHTemp (°C)Agitation (rpm)CMCase activity (U/mL)Cell growth (g/L)
MeasuredPredictedMeasuredPredicted

10 (7.0)0 (37)0 (180) 0.437 3.18
21 (7.6)−1 (33)1 (215) 0.335 2.39
3−1 (6.4)1 (41)−1 (144) 0.512 1.85
4α (8.0)0 (37)0 (180) 0.503 2.53
50 (7.0)0 (37)α (120) 0.490 2.14
6 (5.9)0 (37)0 (180) 0.360 2.10
70 (7.0)0 (37)0 (180) 0.437 3.18
80 (7.0)0 (37)0 (180) 0.437 3.18
90 (7.0)0 (37)0 (180) 0.437 3.18
101 (7.6)1 (41)−1 (144) 0.538 2.06
111 (7.6)1 (41)1 (215) 0.374 2.93
120 (7.0)0 (37)0 (180) 0.437 3.18
13−1 (6.4)−1 (33)−1 (144) 0.202 1.75
140 (7.0)0 (37)0 (180) 0.437 3.18
151 (7.6)−1 (33)−1 (144) 0.325 2.01
16−1 (6.4)1 (41)1 (215) 0.327 2.67
170 (7.0)0 (37)α (240) 0.344 3.15
180 (7.0)α (44)0 (180) 0.365 2.13
190 (7.0)α (30)0 (180) 0.071 1.58
20−1 (6.4)−1 (33)1 (215) 0.192 2.08

Values are mean ± SE ( ).