Enhanced Cellulase Production from Bacillus subtilis by Optimizing Physical Parameters for Bioethanol Production

Table 1

CCD showing experimental and regression model predicted CMCase activity (U/mL) and cell growth (g/L).

Run no.	pH	Temp (°C)	Agitation (rpm)	CMCase activity (U/mL)		Cell growth (g/L)
				Measured	Predicted	Measured	Predicted
1	0 (7.0)	0 (37)	0 (180)	0.429±0.05	0.437	3.16±0.01	3.18
2	1 (7.6)	−1 (33)	1 (215)	0.311±0.01	0.335	2.30±0.05	2.39
3	−1 (6.4)	1 (41)	−1 (144)	0.499±0.02	0.512	1.87±0.12	1.85
4	α (8.0)	0 (37)	0 (180)	0.560±0.01	0.503	2.74±0.09	2.53
5	0 (7.0)	0 (37)	−α (120)	0.500±0.08	0.490	2.08±0.01	2.14
6	−𝛼 (5.9)	0 (37)	0 (180)	0.355±0.05	0.360	1.98±0.04	2.10
7	0 (7.0)	0 (37)	0 (180)	0.442±0.02	0.437	3.23±0.14	3.18
8	0 (7.0)	0 (37)	0 (180)	0.436±0.03	0.437	3.19±0.06	3.18
9	0 (7.0)	0 (37)	0 (180)	0.422±0.06	0.437	3.16±0.02	3.18
10	1 (7.6)	1 (41)	−1 (144)	0.532±0.02	0.538	2.08±0.08	2.06
11	1 (7.6)	1 (41)	1 (215)	0.322±0.02	0.374	2.73±0.05	2.93
12	0 (7.0)	0 (37)	0 (180)	0.448±0.01	0.437	3.14±0.04	3.18
13	−1 (6.4)	−1 (33)	−1 (144)	0.217±0.07	0.202	1.88±0.02	1.75
14	0 (7.0)	0 (37)	0 (180)	0.435±0.04	0.437	3.19±0.21	3.18
15	1 (7.6)	−1 (33)	−1 (144)	0.281±0.11	0.325	1.87±0.05	2.01
16	−1 (6.4)	1 (41)	1 (215)	0.334±0.01	0.327	2.73±0.02	2.67
17	0 (7.0)	0 (37)	α (240)	0.386±0.05	0.344	3.30±0.04	3.15
18	0 (7.0)	α (44)	0 (180)	0.385±0.01	0.365	2.15±0.18	2.13
19	0 (7.0)	−α (30)	0 (180)	0.103±0.14	0.071	1.65±0.05	1.58
20	−1 (6.4)	−1 (33)	1 (215)	0.161±0.03	0.192	2.00±0.01	2.08

Values are mean ± SE (𝑛=3).