Enhanced Cellulase Production from Bacillus subtilis by Optimizing Physical Parameters for Bioethanol Production
Table 2
(a) Analysis of variance (ANOVA) for alkaline CMCase activity (U/mL) in the optimization study, (b) analysis of variance (ANOVA) for cell growth (g/L) of Bacillus subtilis AS3 in the optimization study.
(a)
Source
df
SS
Adj MS
Regression
9
0.261363
0.029040
21.18
0.000
95.02
Linear
3
0.154385
0.051462
37.54
0.000
Square
3
0.086877
0.028959
21.13
0.000
Interaction
3
0.020102
0.006701
4.89
0.024
Residual error
10
0.013708
0.001371
Pure error
5
0.000404
0.000081
Total
19
0.275071
(b)
Source
df
SS
Adj MS
Regression
9
6.30943
0.70105
37.48
0.000
97.12
Linear
3
1.82418
0.60806
32.51
0.000
Square
3
4.36800
1.45600
77.85
0.000
Interaction
3
0.11725
0.03908
2.09
0.165
Residual error
10
0.18702
0.01870
Pure error
5
0.00508
0.00102
Total
19
6.49645
df: degrees of freedom; SS: sum of squares; MS: mean sum of squares. : Fisher’s value (calculated by dividing the MS owing to the model by that due to error); : probability of incorrectly rejecting the null hypothesis when it is actually true.
