Insulin Deprivation Decreases Caspase-Dependent Apoptotic Signaling in Cultured Rat Sertoli Cells

Table 1

Oligonucleotides and cycling conditions for PCR amplification of p53, bcl-2, Bax, caspase 9, caspase 3, and 18S.

Gene	Primer sequences	AT	C	Size (bp)
p53	Forward—CTG CCC ACC ACA GCG ACA GG	59°C	35	471
	Reverse—AGG AGC CAG GCC GTC ACC AT
Bcl-2	Forward—GGG CTA CGA GTC GGA TAC	53°C	35	64
	Reverse—AGG CTG GAA GGA GAA GAT G
Bax	Forward—CGC GTG GTT GCC CTC TTC TAC TTT	59°C	35	124
	Reverse—CAA GCA GCC GCT CAC GGA GGA
Caspase 9	Forward—TGC AGG GTA CGC CTT GTG CG	61°C	35	130
	Reverse—CCT GAT CCC GCC GAG ACC CA
Caspase 3	Forward—AGG CCT GCC GAG GTA CAG AGC	61°C	35	255
	Reverse—CCG TGG CCA CCT TCC GCT TA
18s	Forward—AAG ACG AAC CAG AGC GAA AG	56°C	25	149
	Reverse—GGC GGG TCA TGG GAA TAA

AT: annealing temperature; C: number of cycles during exponential phase of amplification.