Thymic Stromal Lymphopoietin Enhances Th2/Th22 and Reduces IL-17A in Protease-Allergen-Induced Airways Inflammation

Figure 8

Reduced pulmonary IL-5⁺ and IL-22⁺ cell populations in the absence of TSLPR. Lung mononuclear cells from OVA sensitized and challenged WT and TSLPR^−/− mice were isolated and restimulated for 4 h with PMA (50 ng/mL) and ionomycin (750 ng/mL) followed by extracellular staining of TCR𝛾𝛿 and CD4. Representative dot plot, the frequency and the absolute numbers of IL-5⁺ and IL-22⁺ producing cells gated on TCR𝛼𝛽⁺CD4⁺ T cell populations (a–e) are shown. Values are the mean ± SEM of 6 mice per group.

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