Comparison of Lipase Production by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361 Using Fish Waste as Substrate: Optimization of Culture Conditions by Response Surface Methodology
Table 2
Factorial design for screening of significant independent variables affecting lipase production with the observed lipase activity values.
pH
temp (°C)
time (h)
FWPH (mg/mL)
FWO (%v/v)
(U/mL)
(U/mL)
5
37
24
0.1
1
645
97.33
5
37
24
0.1
5
983
269.56
5
37
24
0.2
1
865.976
73.54
5
37
24
0.2
5
1428
391
5
37
72
0.1
1
243.98
41.2
5
37
72
0.1
5
1109.77
215.23
5
37
72
0.2
1
639
51.823
5
37
72
0.2
5
1208.67
287.45
5
47
24
0.1
1
908.743
158
5
47
24
0.1
5
1139.89
276
5
47
24
0.2
1
1097
89.779
5
47
24
0.2
5
1565.09
234
5
47
72
0.1
1
367.8188
68.9
5
47
72
0.1
5
890.098
213.856
5
47
72
0.2
1
737.51
49
5
47
72
0.2
5
1183
330
7
37
24
0.1
1
410
89.12
7
37
24
0.1
5
1430
274.33
7
37
24
0.2
1
1123
121
7
37
24
0.2
5
1603.45
409.66
7
37
72
0.1
1
378
34.567
7
37
72
0.1
5
967
208
7
37
72
0.2
1
809.09
58.526
7
37
72
0.2
5
1118
281
7
47
24
0.1
1
1010
91.23
7
47
24
0.1
5
1324
299
7
47
24
0.2
1
1238.45
145.89
7
47
24
0.2
5
1365.23
391.9
7
47
72
0.1
1
733
89.98
7
47
72
0.1
5
889
195
7
47
72
0.2
1
900.23
96
7
47
72
0.2
5
1203
310
: pH; 2: temperature, °C; 3: time, hours; 4: FWPH concentration (mg/mL); 5: FWO concentration, % v/v; : lipase activity (U/mL) of MTCC 5695; : lipase activity of MTCC 11361; FWPH: fish waste protein hydrolysates; FWO: fish waste oil.
